DNA

Part:BBa_K5300044:Design

Designed by: Fan Yaxu   Group: iGEM24_CAU-China   (2024-09-30)


PnuoA-Cas12k-double Terminator-PglnK-sgRNA-GFP-tracRNA-PnifH-gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4445
    Illegal AgeI site found at 5
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3850


Design Notes

None


Source

Sinorhizobium fredii CCBAU45436, Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110), Scytonema hofmannii.

References